Integrated DNA & RNA Profiling
Comprehensive Genomic Insights for Precision Oncology
DNA GTx’s TruSight Oncology 500 is a powerful, next-generation sequencing panel that analyzes DNA from 523 genes and RNA from 55 genes using formalin-fixed, paraffin-embedded (FFPE) tumor samples. This dual-analysis approach enables detection of somatic mutations, gene fusions, and transcriptional changes, delivering a holistic view of tumor biology to support personalized cancer treatment decisions.
Who is this Test for?
- Enrollment in clinical trials requiring detailed molecular characterization
- Immunotherapy consideration (requires biomarkers like TMB/MSI)
- Cases where previous panel was inconclusive or negative
Variant Types Detected
Single Nucleotide Variants (SNVs)
- SNVs are single base changes between reference and the patient's genome.
Insertions and Deletions (Indels)
- Insertion
- Deletion
- Indels are small changes in DNA where a few bases are either added (insertion) or removed (deletion).
Copy Number Variants (CNVs)
- Amplification / Duplication
- Deletion
- Parts of DNA that are copied too many times or missing. This can make cancer genes overactive or silence protective genes.
Microsatellite Instability (MSI)
- Microsatellites are short, repeated DNA sequences (like “stutters” in the genetic code). Normally, cells fix mistakes in these repeats. If the DNA repair system is broken, the repeats become unstable. It’s an important marker for certain cancers and can predict response to immunotherapy.
Tumor Mutational Burden (TMB)
- TMB is the total number of mutations in a tumor’s DNA. A high TMB means the tumor has many genetic changes, which can make it easier for the immune system to recognize and attack it, and may predict response to immunotherapy.
What it Screens for
- Mutations in 523 cancer-related genes (DNA)
- Gene fusions and expression changes in 55 genes (RNA)
- Genetic alterations linked to:
- Lung cancer
- Colorectal cancer
- Breast cancer
- Ovarian cancer
- Melanoma
- Other solid tumors
Show list
- Pancreatic cancer
- Prostate cancer
- Bladder cancer
- Gastric (stomach) cancer
- Esophageal cancer
- Endometrial (uterine) cancer
- Thyroid cancer
- Head and neck cancers
- Neuroendocrine tumors
- Soft tissue sarcomas
Key Benefits
- Comprehensive Analysis: Analyzes both DNA mutations and RNA changes for a complete tumor profile.
- Detects Multiple Alterations: Identifies mutations, gene fusions, and gene expression changes.
- Supports Personalized Treatment: Provides detailed insights to guide tailored cancer therapies.
Turn Around Time
- 5-6 weeks
Do You Need a Doctor’s Request?
Yes, a physician’s order is required. This is a complex diagnostic test with clinical implications for targeted therapy, immunotherapy, and trial enrollment. A licensed healthcare provider must assess eligibility and interpret the results as part of the patient’s treatment plan.
How it Works?
Step 1: FFPE Tissue Sample Collection
- Preserved tissue sample are collected from biopsies or surgeries.
- Stored in wax blocks, these samples keep DNA intact for testing.
Step 2: DNA & RNA Extraction
- We carefully isolate both DNA and RNA from the tissue.
- This allows us to study both genetic changes and gene activity.
Step 3: Library Preparation
- The DNA is prepared so it can be read by our sequencing machines.
- We add special tags and focus on the most important parts of your DNA.
Step 4: Next Generation Sequencing
- This is how we ‘’read’’ your DNA using advanced technology.
- It helps us find changes or mutations in your genes.
Step 5: Data Analysis
- Our proprietary software (D-KRYPT) helps us understand what the DNA changes mean.
- The results are derived from trusted medical databases.
Step 6: Personalized Patient Report
- You got a clear, easy-to-read report with important findings.
- Your doctor can use this to guide treatment or next steps.
Oncology Test Panels
Séquençage clinique
Solutions for Researchers
Prise en charge prénatale
Médecine régénérative
Healthcare
Agriculture
Expand Your Oncology Insights
With 523 DNA and 55 RNA targets, TruSight Oncology 500 delivers comprehensive tumor characterization from FFPE samples.
*All information is handled with strict confidentiality.
A Deeper Look Into Your Cancer
The TruSight Oncology 500 (DNA & RNA) test examines both DNA and RNA in tumor tissue to provide your doctor with a clearer picture of what drives your cancer.
*All information is handled with strict confidentiality.
